A comparative analysis on serum antibody levels of sheep immunized with crude and thiol-purified excretorysecretory antigen of Haemonchus contortus
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چکیده
منابع مشابه
A comparative analysis on serum antibody levels of sheep immunized with crude and thiol-purified excretory/secretory antigen of Haemonchus contortus
In vitro culture of live adult worms were made in RPMI 1640 medium at a concentration of approximately 50 worms / ml in a 0 culture flask at 37 C for 24 hours and the culture supernatant was used as antigen. The E/S antigen was purified by thiol – sepharose affinity chromatography. On western blot analysis, it was demonstrated that the crude E/S antigen showed five reactive bands at 24, 29, 46,...
متن کاملImmunoprotection in sheep against Haemonchus contortus using its thiol-purified excretory/secretory proteins
Excretory/Secretory antigen was prepared by culturing live adult worms of Haemonchus contortus in RPMI 1640 medium at a concentration of 50 worms per mL in a culture-flask at 37 ˚C for 24 hr and the culture supernatant was used as antigen. The E/S antigen was purified by thiol-sepharose affinity chromatography. On western blot analysis, it was demonstrated that thiol-purified antigen showed a s...
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Immunoprotection in sheep against Haemonchus contortus using its thiol-purified excretory/secretory proteins
Excretory/Secretory antigen was prepared by culturing live adult worms of Haemonchus contortus in RPMI 1640 medium at a concentration of 50 worms per mL in a culture-flask at 37 ˚C for 24 hr and the culture supernatant was used as antigen. The E/S antigen was purified by thiol-sepharose affinity chromatography. On western blot analysis, it was demonstrated that thiol-purified antigen showed a s...
متن کاملimmunoprotection in sheep against haemonchus contortus using its thiol-purified excretory/secretory proteins
excretory/secretory antigen was prepared by culturing live adult worms of haemonchus contortus in rpmi 1640 medium at a concentration of 50 worms per ml in a culture-flask at 37 ˚c for 24 hr and the culture supernatant was used as antigen. the e/s antigen was purified by thiol-sepharose affinity chromatography. on western blot analysis, it was demonstrated that thiol-purified antigen showed a s...
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ژورنال
عنوان ژورنال: Veterinary World
سال: 2012
ISSN: 0972-8988
DOI: 10.5455/vetworld.2012.279-284